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Bioconversion of sawdust into ethanol using dilute sulfuric acid-assisted continuous twin screw-driven reactor pretreatment and fed-batch simultaneous saccharification and fermentation.

Identifieur interne : 002782 ( Main/Exploration ); précédent : 002781; suivant : 002783

Bioconversion of sawdust into ethanol using dilute sulfuric acid-assisted continuous twin screw-driven reactor pretreatment and fed-batch simultaneous saccharification and fermentation.

Auteurs : Tae Hyun Kim [Corée du Sud] ; Chang Ho Choi ; Kyeong Keun Oh

Source :

RBID : pubmed:23306134

Descripteurs français

English descriptors

Abstract

Ethanol production from poplar sawdust using sulfuric acid-assisted continuous twin screw-driven reactor (CTSR) pretreatment followed by simultaneous saccharification and fermentation (SSF) was investigated. Pretreatment with high acid concentration increased the cellulose content in the pretreated solid (74.9-76.9% in the range of 4.0-5.5wt.% H(2)SO(4)). The sugar content (XMG; xylan+mannan+galactan) in the treated-solid was 11.1-15.2% and 0.9-5.7% with 0.5wt.% and 7.0wt.%, respectively. The XMG recovery yield of the sample treated with 4.0wt.% H(2)SO(4) at 185°C was maximized at 88.6%. Enzymatic hydrolysis test showed a cellulose digestibility of 67.1%, 70.1%, and 73.6% with 15, 30, and 45FPU/g-cellulose, respectively. In the fed-batch SSF tests with initial enzyme addition, the ethanol yield of each stage almost reached a maximum at 28h, 48h, and 56h, respectively, with yields of 63.9% (16.5g/L), 78.4% (30.1g/L), and 81.7% (39.9g/L), respectively.

DOI: 10.1016/j.biortech.2012.11.125
PubMed: 23306134


Affiliations:


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Le document en format XML

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<term>Ethanol (isolation & purification)</term>
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<div type="abstract" xml:lang="en">Ethanol production from poplar sawdust using sulfuric acid-assisted continuous twin screw-driven reactor (CTSR) pretreatment followed by simultaneous saccharification and fermentation (SSF) was investigated. Pretreatment with high acid concentration increased the cellulose content in the pretreated solid (74.9-76.9% in the range of 4.0-5.5wt.% H(2)SO(4)). The sugar content (XMG; xylan+mannan+galactan) in the treated-solid was 11.1-15.2% and 0.9-5.7% with 0.5wt.% and 7.0wt.%, respectively. The XMG recovery yield of the sample treated with 4.0wt.% H(2)SO(4) at 185°C was maximized at 88.6%. Enzymatic hydrolysis test showed a cellulose digestibility of 67.1%, 70.1%, and 73.6% with 15, 30, and 45FPU/g-cellulose, respectively. In the fed-batch SSF tests with initial enzyme addition, the ethanol yield of each stage almost reached a maximum at 28h, 48h, and 56h, respectively, with yields of 63.9% (16.5g/L), 78.4% (30.1g/L), and 81.7% (39.9g/L), respectively.</div>
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